Zynt Bio develops a cloud-native bioinformatics platform optimized exclusively for plant systems, host-pathogen dynamics, environmental metagenomics, and industrial biomanufacturing. By deploying containerized Nextflow workflows on AWS, we deliver reproducible, audited, and scalable data processing.
Platform Roadmap (In Active Development)
Zynt Bio is standardizing computational genomics into modular, cloud-native automated engines. The following analytical pipelines are actively under development on AWS Batch, designed to seamlessly scale our platform offerings as our underlying infrastructure expands:
Zynt DualSeq™
A specialized host-pathogen transcriptomic pipeline designed to simultaneously align and de-convolve mixed sequence matrices, decoupling plant immune responses from fungal or bacterial virulence factors.
Zynt ResistAMP™
A comprehensive metagenomic pipeline utilizing advanced profiling to simultaneously screen environmental and agricultural datasets for antimicrobial resistance genes (AMR/ARGs) alongside novel antimicrobial peptides (AMPs).
Core Platform Processing Architecture
1
Functional Transcriptomics & Regulatory RNA-Seq
High-resolution quantification and profiling of coding and non-coding RNA dynamics.
- DGE & Modeling: High-throughput transcript abundance estimation across multi-factor experimental designs.
- Small RNA & RNAi Profiling: Precision identification, size distribution, and target prediction for regulatory sRNA and silencing constructs.
- Functional Enrichment: GO terms, KEGG pathways, and metabolic network mapping.
2
Host-Pathogen Cross-Talk
Specialized containerized workflows to separate host, vector, and microbial sequence profiles in mixed infections.
- Dual RNA-Seq Processing: Simultaneous alignment and transcript de-convolution to decouple plant immunity responses from pathogen virulence.
3
Soil & Environmental Metagenomics
Shotgun and amplicon workflows for diverse soil and plant-associated microbiomes.
- MAG Binning: High-throughput assembly and advanced binning (MetaBAT2, MaxBin2) to reconstruct high-quality Metagenome-Assembled Genomes.
- Amplicon Profiling: Optimized taxonomic profiling using 16S, ITS, and 18S target regions.
- Resistome Profiling: Environmental AMR and antibiotic resistance gene detection from agricultural data.
4
Industrial Biotech Strain Verification
Automated auditing tailored for synthetic biology, crop protection, and biomanufacturing.
- BGC Mining: Genome and metagenome mining for novel biosynthetic gene clusters and pathways.
- Resequencing & Variants: High-coverage alignment to detect SNPs, indels, and structural variations.
- CRISPR Screening: Rigorous genome-wide scanning for off-target edits.
- Cassette Audits: Verification ensuring synthetic constructs match exact design specifications.
Deliverables Included with Every Project
- Synthesis Portfolio: Executive and technical reports interpreting biological relevance.
- Methodology Documentation: Formal narratives detailing parameters, databases, and steps.
- R&D Reporting: Data ingestion criteria, alignment constraints, and validation thresholds.
- Traceability Logs: Full workflow metadata, container hashes, and Nextflow execution logs.
- Secure Cloud Assets: Cleaned FASTQs, BAM alignments, and count matrices archived in isolated S3 buckets.
